SENSISpec INgezim Gluten R5 96 wells

The SENSISpec INgezim Gluten kit provides fast and reliable quantitative results for gluten detection.

SENSISpec INgezim Gluten R5 ELISA – AOAC Performance Tested Method

The assay is based on the R5 Monoclonal Antibody, which is specific for proteins from wheat, rye and barley. The Limit of Detection of the assay is 3 ppm (mg/kg), lower than the limits established by the Codex Alimentarius.

The AOAC Performance Tested Method status of the assay demonstrates that after a rigorous validation study, all the results obtained were satisfactory according with the expected.

The results confirm the consistency of the kit to be used as a reliable tool within an allergen risk management program and a validated assay for ISO 17025 accredited laboratories.The SENSISpec INgezim Gluten R5 kit provides fast and reliable quantitative results for gluten detection.

WATCH THIS VIDEO to learn about the procedure of the SENSISpec INgezim Gluten kit, including automation using the ThunderBoltTM ELISA processing instrument.



Gluten intolerance is characterized by adverse reactions to gluten, a group of proteins found in wheat, barley and rye. People with celiac disease or gluten sensitivity need to avoid gluten containing foods that trigger symptoms.

The SENSISpec Ingezim Gluten R5 Test Kit uses the R5 monoclonal antibody for specific detection of prolamin sequences from wheat, rye and barley. Research studies have shown that the R5 antibody specifically targets the component of the gluten fraction that is believed to be directly responsible for
celiac disease.

With its limit of quantification of 3 ppm gluten the SENSISpec Ingezim Gluten R5 Test Kit is one of the most sensitive test kits in the market, creating additional safety for consumers with celiac disease.

The kit is provided in a 96 well format and includes all necessary reagents including extraction solution.


Visit our Youtube Channel and watch this video to learn about the procedure of the SENSISpec INgezim Gluten kit, including automation using the ThunderBoltTM ELISA processing instrument.

Interested in more details? This WEBINAR VIDEO includes a detailed presentation from our allergen product expert and an exciting Q&A session.

Kit Specifications
Test principle
Sandwich enzyme immunoassay
Microtiter plate with 96 wells
Incubation Time
130 min (60/60/10 min)
25; 12.5; 6.25; 3.12; 1.56 ng/mL (European Gliadin)
450 nm
Detection limit (LOD)
3 ppm of gluten
Quantitation Range
3-200 ppm
Shelf Life (months)
Product Attachments
  • AOAC Certification_22C_052005_INgenzimGluR5.pdf

  • IFU_P30GLUK2 SENSISpec INgezim GLUTEN R5_mod.pdf

  • IT-I-30-GLUK2 I-technical report INGEZIM GLUTEN_2018.pdf

  • SENSISpec INgezim Gluten R5 DATA EVALUATION SHEET (Version 03-2022)