Immunoassay for the quantitative and sensitive screening of Melamine.
The Melamine ELISA is an immunoassay for the quantitative and sensitive screening of Melamine. This test is suitable for the quantitative and/or qualitative screening of Melamine in various sample matrices (please contact Abraxis for the appropriate extraction/dilution procedure technical bulletins). If necessary, samples requiring regulatory action can be confirmed by HPLC, GC/MS, or other conventional methods.
The test is based on the recognition of Melamine by antibodies. The standards, sample extracts, and Melamine HRP conjugate are pipetted into test wells coated with Melamine antibody to initiate the reaction. During the 30 minute incubation period, Melamine from the sample and Melamine HRP conjugate compete for binding to Melamine antibody. Following this 30 minute incubation, the contents of the well are removed and the wells are washed to remove any unbound Melamine and Melamine HRP conjugate. After washing with the diluted wash solution, a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 20 minute incubation, the reaction is stopped and the amount of color in each well is read using an ELISA reader. The color of the unknown samples is compared to the color of the standards and the Melamine concentration of the samples is derived. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.