Immunoassay for the quantitative or qualitative detection of Trifluralin.
The Trifluralin ELISA is an immunoassay for the detection of Trifluralin in surface water, fish, or shrimp. This test is suitable for the quantitative or qualitative detection of Trifluralin in contaminated samples. Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Trifluralin by specific antibodies. Trifluralin, when present in a sample and a Trifluralin-enzyme conjugate compete for the binding sites of anti-Trifluralin antibodies in solution. The Trifluralin antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Trifluralin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using a microplate ELISA photometer. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.