The Eurofins Technologies Gluten test is based on the principle of immunoassay. Gluten containing sample is given into a reactions vial containing an activation reagent. After 3 minutes incubation at room temperature a test strip is placed into the reaction vial. The sample migrates along the nitrocellulose membrane by capillary forces. Along its way it releases gold nanoparticles conjugated to anti-gluten-antibodies. For positive samples a red line is formed when the liquid reaches the test line area. In case of negative samples, no line is formed. In any case, above the test line area a red control line appears, indicating the validity of the test. The test is evaluated after another 5 minutes.
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