Immunoassay for the quantitative and sensitive detection of Anabaenopeptins in water samples.
The Anabaenopeptins ELISA is an immunoassay for the quantitative and sensitive detection of Anabaenopeptins in water samples. This test is suitable for the quantitative and/or qualitative detection of Anabaenopeptins in water samples. If necessary, positive samples can be confirmed by LC/MS, protein phosphatase assay, or other conventional methods. The test is a direct competitive ELISA for the detection of Anabaenopeptins. It is based on the recognition of Anabaenopeptins by specific antibodies. Toxin, when present in a sample, and an Anabaenopeptins-HRP analogue compete for the binding sites of the rabbit anti-Anabaenopeptins antibodies in solution. The anti-Anabaenopeptins antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and an addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Anabaenopeptins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.