Immunoassay for the detection of Imidacloprid and other neonicotinoid insecticides.
The Imidacloprid ELISA is an immunoassay for the detection of Imidacloprid and other neonicotinoid insecticides. This test is suitable for the quantitative and/or qualitative detection of Imidacloprid and other neonicotinoids in contaminated samples. Positive samples should be confirmed by GC/MS, HPLC, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Imidacloprid by specific antibodies. Imidacloprid, when present in a sample and an Imidacloprid-enzyme conjugate compete for the binding sites of anti-Imidacloprid antibodies which are immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Imidacloprid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.