Phthalates, ELISA, 96-test

Immunoassay for the detection of Phthalates in surface water.

Online purchase of the environmental & water products is available only from Europe or Asia.
US customers shall contact Gold Standard Diagnostics Horsham directly via e-mail or phone +1-215-357-3911.

This is a made to order item, a minimum order quantity of 5 kits is required to initiate production.


The Phthalates ELISA is an immunoassay for the detection of Phthalates in surface water. This test is suitable for the quantitative and/or qualitative detection of Phthalates in contaminated samples. Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods.

The test is a direct competitive ELISA based on the recognition of Phthalates by specific antibodies. Phthalates, when present in a sample, and a Phthalates-enzyme conjugate compete for the binding sites of anti-Phthalates antibodies in solution. The Phthalates antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Phthalates present in the sample. The color reaction is stopped after a specified time and the color is evaluated using a microplate ELISA photometer. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

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