Immunoassay for the quantitative and sensitive detection of Caffeine.
"The Caffeine ELISA is an immunoassay for the quantitative and sensitive detection of Caffeine. This test is suitable for the quantitative and/or qualitative screening of Caffeine in water, urine, and saliva samples. If necessary, positive samples can be confirmed by HPLC, GC/MS, or other conventional methods. Note: This assay is intended For Research Use Only. The test is a direct competitive ELISA which is based on the recognition of Caffeine by specific antibodies.
Caffeine, when present in a sample, and a Caffeine-HRP analogue compete for the binding sites of mouse anti-Caffeine antibodies in solution.
The Caffeine antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Caffeine present in the sample.The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run."