ELISA test kit for the quantitative detection of corticosteroids
Details
ELISA test kit for the quantitative detection of corticosteroids
Kit Specifications
SKU
HU0050023
Assay principle
Microplate competitive enzyme immunoassay.
Format
Microtiter plate with 96 wells
Assay time
105 min
Analysable samples
Muscle, Urine, Liver
Detection limit (LOD)
- Muscle, Liver - 0.025 ppb
- Urine - 0.25 ppb
Sample preparation
- Urine: centrifugation, dilution, enzymatic hydrolysis, purification with C18 columns, evaporation, reconstitution
- Muscle: homogenization, solvent extraction, centrifugation, evaporation, reconstitution, purification with C18 column
- Liver: homogenization, enzymatic hydrolysis, solvent extraction, centrifugation, evaporation, reconstitution, centrifugation, purification with C18 column.
Specificity
- Zeranol 100%
- Zearalanone 522%
- Taleranol (β-zearalanol) 110%
- α-zearalenol 61%
- β-zearalenol 6.8%
- HEX <0.05%
- DES <0.05%
- Dienestrol <0.05%
- 17-β-estradiol <0.05%
- Progesterone <0.05%
- Testosterone <0.05%
- Clenbuterol <0.05%
- Trenbolone <0.05%
- Dexamethasone <0.05%