ELISA test kit for the quantitative detection of chloramphenicol.
Details
ELISA test kit for the quantitative detection of chloramphenicol.
Kit Specifications
SKU
HU0050002
Assay principle
Microplate competitive enzyme immunoassay.
Format
Microtiter plate with 96 wells
Assay time
45 min
Analysable samples
Urine, Water, Serum and Plasma, Tissue, Honey, Seafood, Egg
Detection limit (LOD)
- Urine (Method II), Serum, Plasma, Water - 0.1 ppb
- Urine (Method I) - 10 ppb
- Honey, Egg - 0.05 ppb
- Muscle, Seafood - 0.025 ppb
Sample preparation
- Urine (Method I) - Centrifugation, Dilution
- Urine (Method II) - pH adjustment, enzymatic hydrolysis, solvent extraction, evaporation, reconstitution
- Water: pH adjustment, dilution (optional)
- Serum & Plasma - Solvent extraction, centrifugation, evaporation, reconstitution
- Eggs, tissue and seafood - homogenization, solvent extraction, centrifugation, evaporation, reconstitution
- Honey - Dilution, solvent extraction centrifugation, evaporation, reconstitution
Specificity
- Chloramphenicol 100%
- Chloramphenicol Glucuronide 70%
- Florophenicol <0.1%
- Tiamphenicol <0.1%