INgezim ASF CROM Ab 2.0 (100 tests)

African Swine Fever (ASF) is a highly contagious viral disease that affects domestic and wild pigs, causing severe economic losses in the swine industry. There is currently no effective vaccine, so rapid detection and control are essential to prevent its spread.

INgezim^® ASFV CROM Ab 2.0 is a qualitative lateral flow or immunochromatographic assay based on the use of colloidal gold nanoparticles bound to a recombinant form of the viral protein VP72 of African Swine Fever Virus (ASFV) or to a control protein. The membrane contains a test line region (T) in which that same form of the VP72 is immobilised and a control line region (C) in which an antibody to the control protein is immobilised. If the sample contains antibodies to ASFV, they will interact with the protein bound to the gold nanoparticles. After adding the running buffer, these gold-protein-antibody complexes will migrate through the membrane and they will bind to the VP72 protein immobilised in the T region, resulting in the appearance of a reddish coloured line. In case no antibodies are present in the sample, no colour will appear in that region.

In both cases, colloidal gold nanoparticles covered with the control protein will continue to migrate through the membrane and they will be recognised by the monoclonal antibody immobilised in the control line region. For this reason, the appearance of a red line in the control line region (C) indicates that the assay developed correctly.v

The test can be performed using the following samples collected from swine (pigs or wild boar): Fresh blood samples and blood samples collected by venous puncture.