INgene q PPA Probes is a real-time PCR kit suited for the detection of DNA of African Swine Fever Virus (ASFV ) in biological samples in a simple way, with high levels of sensitivity and specificity.
The kit contains all necessary reagents and enzymes in two mixes. When mixed together, they are at the required concentrations designed to detect (and optionally quantify) ASFV, simply by adding the DNA of the problem sample. This kit makes use of a UPL® probe labelled with FAM. On the other hand, each Master Mix provides a positive internal control (PIC) with primers and a Taqman probe marked with VIC, allowing detection of false negatives due to an inhibition of the PCR. In addition, the master mix contains a passive reference fluorochrome, ROX, allowing the normalisation of the signal avoiding possible quantification errors deriving from pipetting differences. When using Applied Biosystems' ABI PRISM PCR models in real time, this ROX is necessary to prevent interferences between the data collected from various emission channels. The kit includes an ASFV positive control for detection. The kit doesn´t include a standard positive control with a known number of ASFV copies (ask our commercial department for standard control). This control allows generating a standard curve which links the number of pathogen copies with the cycle threshold (Ct) value.
The kit has been tested for every system from Applied Biosystems (StepOne, HT7300, HT7500) and LC480 from Roche. It is possible to work with other thermocyclers from other commercial houses, if they have at least two fluorescence channels, but Ct values may experiment changes.
Visit our Youtube Channel and watch this video to learn about the procedure of the INgene q PPA kit assay.