Ingezim® ASF CROM Ag is an immunochromatographic assay for the detection of ASFV in blood samples. The test is based on the use of two different coloured latex microspheres: black microspheres that are coated with a specific MAb to ASFV, and blue microspheres, which are used as test control.
On the membrane, two lines are printed: the test line (T) has a specific MAb to ASFV and the control line (C) has a specific MAb for the control protein.
If the case of a positive sample, the virus binds to the black beads conjugated to an anti-ASFV MAb. The immune complex then migrates through the membrane by capillarity and is captured again by the anti-AFSV MAb absorbed on the test line, resulting in the appearance of a black line. The presence of the control line serves as validity of the test, indicating that the immunochromatography has been performed correctly.
Visit our Youtube Channel and watch this video to learn about the procedure of the Ingezim® ASF CROM Ag assay.