The kit is used for the qualitative detection (yes/no) of Cry1Ac and Cry2A Proteins present in Bollgard II in genetically modified individual cotton seed or leaf. The total incubation time of the test is 10 minutes.
An antibody specific to Cry1Ac and Cry2A immobilized on the nitrocellulose membrane as two different test lines. An anti-mouse lgG is immobilized as control line, Second antibody specific for Cry1Ac and Cry2A is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. When the LFS is placed in the sample extract, the Cry1Ac or Cry2A protein, if present in the sample extracts binds to the corresponding antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the membrane as two different test lines resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of protein in sample extract (Cry1Ac or Cry2A) the corresponding test line does not appear. Control line always appears in pink/purple color indicating validity of the test protocol.
No. of tests
Max no. of samples
Qualitative detection (yes/no) of Cry1Ac and Cry2A Proteins present in Bollgard II in genetically modified individual cotton seed or leaf.
Individual cotton seed or leaf
2 - 8° C
Cry1Ac or Cry2A strips: 50 strips in one canister:Two canisters per kit; Extraction buffer:Two vials of 50 ml each (Ready to use); One dropper, One pack insert