VIRSeek Murine Norovirus (MNV) Process Control

Murine norovirus spiking material (1 mL) and real-time RT-PCR kit with 48 reactions for rapid detection of murine norovirus (MNV) process control virus in environmental samples. The kit is intended to be used in analytical laboratories and may also be applied for other purposes in food product research and the analytical field, e.g. monitoring of contaminations during production processes.

The VIRSeek Murine Norovirus (MNV) Process Control Real-time RT-PCR kit provides materials for spiking of samples analysed for viruses and a subsequent quantification of the process control virus in order to control the virus isolation and pathogen detection process in food and surface samples. The kit has been validated for a diverse range of matrices including soft fruits, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish as well as on food surfaces, as described in the ISO 15216-2:2019 or environmental surfaces (WHO protocol “Surface sampling of coronavirus disease (COVID-19): A practical “how to” protocol for health care and public health professionals, Version: 1.1, 02/2020).

According to ISO guidelines 15216-2:2019 the processes and horizontal methods for detection of hepatitis A virus (HAV) and norovirus (NoV) using real-time RT-PCR in food samples require the usage of a process control virus in order to monitor the quality of the entire viral extraction procedure. Process control viruses need to have similar morphological and physicochemical properties as the target virus of interest. MNV belongs to the family of Caliciviridae and is genetically related to the non-culturable human noroviruses. MNV is a culturable non-enveloped positive-sense single stranded RNA virus of a similar size to the target viruses. Therefore, MNV can be considered a suitable process control virus for detection of HAV or human noroviruses for example.

Kit Specifications
No. of tests
PCR cycler platforms
Validated for use with the Agilent AriaMxTM, Bio-Rad CFX96 TouchTM and CFX96 TouchTM Deep Well PCR platforms.
Kit components
  • 1x OligoMix MNV*, vial with purple cap with primersand probe for MNV RNA, 530 μL, store at -20 °C ± 2 °C, do not freeze / thaw more than 3 times.
    • 1x BasicMix* VIRSeek Food, vial with white cap, 265 μL, store at -20 °C ± 2 °C, do not freeze/thaw more than 3 times.
      • 1x Murine Norovirus, 1 vial with transparent cap, 1 mL, store at -20 °C ± 2 °C, do not freeze/thaw more than 3 times.
        • 1x Negative Control, vial with transparent cap, 500 μL, store at -20 °C ± 2 °C.
          • 1x Molecular grade water, white bottle, 30 mL, store at room temperature.
Product Attachments
Find product documentation under Document Repository