The BACGene Mplex STEC SEROtype kits are multiplex real-time PCR assays, which have been developed for a fast and reliable detection of three of the Top7 non-O157 STEC serogroups each. In combination with the BACGene E. coli O157:H7 assays these kits enable the detection and identification of all of the Top7 STEC serotypes.
Among STEC, E. coli O157:H7 is the most prominent serotype, however other serotypes are also potentially threatening to human health by expressing stx1 and stx2 (the Top6: O145, O121, O111,O103, O45 and O26). The BACGene Mplex STEC SEROtype1 and STEC SEROtype2 assays provide materials for rapid serotyping of the Top6 non-O157 serotypes: BACGene Mplex STEC SEROtype1: Detection of O111, O103, O26 (in one multiplex reaction) BACGene Mplex STEC SEROtype2: Detection of O145, O121 and O45 (in one multiplex reaction)
The kit is certified by AOAC© Research Institute under the Performance Tested MethodsSM Program for the detection of STEC Top7.