The Microcystins/Nodularins (ADDA) ELISA is an immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples.
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The Microcystins/Nodularins (ADDA) ELISA is an immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples.
It is based on the recognition of Microcystins, Nodularins, and their congeners by specific antibodies. Toxin, when present in a sample, and a Microcystins-protein analog immobilized on the plate compete for the binding sites of the anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a second antibody-HRP label is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
