ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers).
WATCH THIS VIDEO to learn about the procedure of our B ZERO product line of enzyme immunoassays with virtual master-curve.
Details
ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers).
Advantages: B ZERO® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.
Visit our Youtube Channel and watch this video to learn about the procedure of our B ZERO product line of enzyme immunoassays with virtual master-curve.
Kit Specifications
SKU
HU0040028
Assay principle
Microplate competitive enzyme immunoassay without standard curve. Sample quantification through interpolation of inhibition value on virtual curve (master-curve) provided for each batch of kit.
Format
Microtiter plate with 48 wells
Assay time
20 min
Analysable samples
Cereals, feed, DDGS, wheat bran and middlings
Detection limit (LOD)
- Wheat, Maize, Barley, Feed and DDGS - 0.04 ppm
- Durum Wheat - 0.12 ppm
- Wheat Bran and Middlings - 0.24 ppm
Sample preparation
- Cereals (Maize, Wheat), Feed, DDGS - grinding, methanol-water extraction, filtration
- Durum Wheat - grinding, water extraction, centrifugation, dilution
- Wheat Bran and Middlings - water extraction, activated charcoal clean-up, filtration, dilution
Specificity
- Deoxynivalenol 100%
- 3-acetyl-deoxynivalenol >100%
- 3-glucosyl-deoxynivalenol 64%
- 15-acetyl-deoxynivalenol 2%
- Nivalenol <4%