Test kit for the qualitative detection of fish DNA, 96 real-time PCR reactions with IPC
Details
Test kit for the qualitative detection of fish DNA, 96 real-time PCR reactions with IPC
Kit Specifications
SKU
5422211310
No. of tests
96
Time schedule
DNA-extraction 2 h
PCR-reaction 4 h
Total time 6 h
Kit components
- 2x MasterMix QL RT IPC (LR/HR+) GS-P-08.036, Fish
- 650 µL BasicMix QL RT
- 390 µL OligoMix QL RT IPC (LR/HR+)
- 2 x Positive control DNA: Genomic DNA from Codfish
- 1x DNA stabilization buffer, 150 µL (for NTCs)
- 2x ROX™ dye, 23 µL (to be used only for High ROX cyclers)
Detection limit (LOD)
LODabs ≤ 10 copies; LODrel ≤ 0.01%
PCR cycler platforms
Agilent MX3005P/3000P, Agilent Aria MX, ABI7500/Fast and ABI 7900HT
Material & Equipment not included
- water, DNase-free
- Vortex
- Micropipettes (variable 1-1000 µL) and filter tips
- PCR optical tubes or plates 0.2 ml and optical caps or seals
- RT-PCR Thermocycler
Storage
-20°C