Kit for the qualitative real-time PCR detection of porcine DNA (Sus scrofa) in food and feed, for use with low-DNA samples.
Details
Kit for the qualitative real-time PCR detection of porcine DNA (Sus scrofa) in food and feed, for use with low-DNA samples.
Kit Specifications
SKU
5422211810
No. of tests
96
Time schedule
DNA-extraction 2 h
PCR-reaction 4 h
Total time 6 h
Kit components
- MasterMix QL RT IPC (LR) GS-P-08.034 pig HS
- 650 µL BasicMix QL RT IPC (NR)
- 390 µL OligoMix QL RT IPC (LR)
- Positive control DNA, 150 µL
- DNA stabilization buffer II, 150 µL (for NTCs)
Detection limit (LOD)
The relative limit of detection has been validated as 0.0001 % of porcine DNA in other species (total DNA content of 25 ng/rxn, pig DNA in cattle DNA (w/w)).
PCR cycler platforms
Agilent MX3005P/ MX3000P, ABI7500 (Fast) and Roche LC480
Material & Equipment not included
- water, DNase-free
- Vortex
- Micropipettes (variable 1-1000 µL) and filter tips
- PCR optical tubes or plates 0.2 ml and optical caps or seals
- RT-PCR Thermocycler
Storage
-20°C