Lateral-flow Device for the Determination of Lupin in Food and as Cleaning Control Monitoring
The SENSIStrip Lupin test is based on the principle of immunoassay. Lupin containing sample is given into a reactions vial containing biotinylated antibody directed against lupin proteins. After 3 minutes incubation at room temperature a test strip is placed into the reaction vial. The sample migrates along the nitrocellulose membrane by capillary forces. Along its way it releases gold nanoparticles conjugated to streptavidin. An antibody-gold complex is formed. For positive samples a red line is formed when the liquid reaches the test line area. In case of negative samples, no line is formed. In any case, above the test line area a red control line appears, indicating the validity of the test. The test is evaluated after another 5 minutes.
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