mCry3A lateral flow test kit (LFS) is intended to be used for the qualitative detection of modified Cry3A protein present in transgenic Maize plant carrying Syngenta corn root worm event MIR 604. This event is present in Syngenta Agrisure RW. The test is used to detect the mCry3A protein in individual Maize seed or leaf.
An antibody specific to the mCry3A protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for mCry3A molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.
When the LFS is placed in the sample extract, the mCry3A protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of mCry3A, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.