The Microcystins/Nodularins (ADDA) ELISA is an immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples.
The Microcystins/Nodularins (ADDA) ELISA is an immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples. It is based on the recognition of Microcystins, Nodularins, and their congeners by specific antibodies. Toxin, when present in a sample, and a Microcystins-protein analog immobilized on the plate compete for the binding sites of the anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a second antibody-HRP label is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
No. of tests
Max no. of samples
0.10 ppb (μg/L)
Time to Result
1. Microtiter plate (12 X 8 strips) coated with an analog of Microcystins conjugated to a protein 2. Standards: 0, 0.15, 0.40, 1.0, 2.0, 5.0 ppb; 1 mL each 3. Control: 0.75 ± 0.185 ppb, 1 mL, prepared from a secondary source, for use as a Quality Control Standard (QCS) 4. Sample Diluent, 25 mL, for use as a Laboratory Reagent Blank (LRB) and for dilution of samples above the range of the standard curve 5. Antibody Solution, 6 mL 6. Anti-Sheep-HRP Conjugate Solution, 12 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 12 mL 9. Stop Solution, 6 mL