INgezim® PPA Compac 2.0 is a blocking immunonzymatic assay based on the use of a monoclonal antibody specific of VP72 protein of the African Swine Fever Virus (ASFV) and a recombinant protein VP72 as an antigen.
Validated by the EURL (CISA-INIA, Spain) and Approved by the Friedrich-Loeffler-Institut FLI, Germany, FLI-C 161.
African Swine Fever (ASF) is a highly contagious viral disease that affects domestic and wild pigs, causing severe economic losses in the swine industry. There is currently no effective vaccine, so rapid detection and control are essential to prevent its spread.
INgezim® PPA Compac 2.0 is a blocking immunoenzymatic assay designed for the detection of antibodies against the VP72 protein of African Swine Fever Virus. It is intended for use with serum samples from pigs, including domestic pigs and wild boar.
The plate wells were previously sensitized with the target antigen, the vp72 of the African swine fever virus, the major structural protein of the virus with high antigenic capability. The sera to be tested are added to the wells and, after a washing step, a specific monoclonal antibody to the vp72 protein labelled with peroxidase is added. When a serum sample contains specific antibodies against the virus, they will bind to the antigen absorbed on the plate, blocking the binding of the monoclonal antibody. If the serum sample does not contain specific antibodies, the monoclonal antibody labelled with peroxidase will be the one that binds to the coated antigen.
Non-fixed material is eliminated from the plate by several washing steps. Afterwards, the presence of bound monoclonal antibody is visualized by the addition of the chromogenic substrate, which acquires a measurable blue color in the presence of peroxidase. In this way, the appearance of color in the well indicates the absence of specific antibodies to the virus in the assayed serum, while the absence of color indicates the presence of specific antibodies.
