I'screen SULFA, ELISA, 96 det.

ELISA test kit for the detection of sulphonamides.
Details
ELISA test kit for the detection of sulphonamides.
Kit Specifications
SKU
HU0050003
Assay principle
Microplate competitive enzyme immunoassay.
Format
Microtiter plate with 96 wells
Assay time
75 min
Analysable samples
Muscle, Egg, Milk, Honey, Feed
Detection limit (LOD)
  • Muscle, Egg (Method I), Honey (Method III, high sensitivity) - 0.5 ppb
  • Milk (Method I) - 0.25 ppb
  • Muscle, Egg, Milk, Honey (Method II, solvent-free) - 5 ppb
  • Feed - 66.6 ppb
Sample preparation
  • Muscle, Egg, Milk (Method I, High Sensitivity) - Homogenization, (Skimming for milk), Solvent Extraction, Centrifugation, Evaporation
  • Muscle, Egg, Honey (Method II, Solvent Free) - Homogenization, Buffer Extraction (Acid Hydrolysis for Honey), Centrifugation, Filtration
  • Milk (Method II) - Centrifugation, Dilution
  • Honey (Method III) - Acidic Hydrolysis, Purification on SPE Column, Elution, Evaporation, Resuspension
  • Feed - Grinding, Solvent Extraction, Dilution
Specificity
  • Sulfamerazine >100%
  • Sulfamonomethoxine >100%
  • Sulfadiazine 100%
  • Sulfachloropyridazine 100%
  • Sulfamethoxypyridazine (Sulfapyridazine) >100%
  • Sulfamethoxydiazine (Sulfamethoxine, Sulfameter) >100%
  • Sulfadimethoxine >100%
  • Sulfaquinoxaline >100%
  • Sulfathiazole >100%
  • Sulfamethizole >100%
  • Sulfamethazine 100%
  • Sulfamethoxazole 90%
  • Sulfisoxazole 50%
  • Sulfapyridine 36%
  • Sulfadoxine 24%
  • Sulfacetamide 2.5%
  • Sulfaphenazole <2%
  • Sulfabenzamide <2%
  • Sulfaguanidine <0.1%
  • Sulfanilamide <0.1%