I’Screen CAP, ELISA, 96 det.

ELISA test kit for quantitative detection of Chloramphenicol

WATCH THIS VIDEO to learn about the procedure of the I’Screen CAP ELISA kit.

Details

ELISA test kit for quantitative detection of Chloramphenicol


Visit our Youtube Channel and watch this video to learn about the procedure of the I’Screen CAP ELISA kit.

Kit Specifications
SKU
HU0050001
Assay principle
Microplate competitive enzyme immunoassay
Format
Microtiter plate with 96 wells
Assay time
45 min
Analysable samples
Honey, eggs, urine, water, milk, plasma, muscle samples, seafood and yoghurt
Detection limit (LOD)

Urine, water, serum or plasma: 0.1 ppb

Milk: 0.01 ppb

Eggs, honey, yoghurt: 0.05 ppb

Muscle, seafood: 0.025 ppb

Sample preparation

Water, urine (method I), water: pH adjustment, dilution (optional)

Urine (method II): pH adjustment, enzymatic hydrolysis, solvent extraction, evaporation, reconstitution

Serum or plasma: solvent extraction, centrifugation, evaporation, reconstitution

Eggs, tissues, seafood and yoghurt: homogenization, solvent extraction, centrifugation, evaporation, reconstitution

Honey: dilution, solvent extraction, centrifugation, centrifugation, evaporation, reconstitution

Milk: solvent extraction, centrifugation, evaporation, reconstitution

Specificity

Chloramphenicol (CAP) 100%

CAP glucuronide 70%

Florfenicol < 0.1%

Tiamphenicol < 0.1%