Magnetic particle kit for detection of Fluridone.
Magnetic particle kit for detection and quantitation of Fluridone in water (groundwater, surface water, well water). For soil, crop, and food use contact the company for application bulletins and/or specific matrix validation guidelines. The Fluridone Kit applies the principles of enzyme-linked immunosorbent assay (ELISA) to the determination of Fluridone. The sample to be tested is added, along with an enzyme conjugate, to a disposable test tube, followed by paramagnetic particles attached with antibodies specific to Fluridone. At this point, a competitive reaction occurs between the Fluridone which may be in the sample and the enzyme-labeled Fluridone analog for the antibody binding sites on the magnetic particles. The reaction is allowed to continue for twenty (20) minutes. At the end of the incubation period, a magnetic field is applied to hold in the test tube the paramagnetic particles (with Fluridone and labeled Fluridone bound to the antibodies on the particles, in proportion to their original concentration), and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of Fluridone is detected by adding the “Color Solution”, which contains the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'-tetramethyl-benzidine). The enzyme-labeled Fluridone bound to the Fluridone antibody catalyzes the conversion of the substrate/chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of a diluted acid (Stopping Solution). Since the labeled Fluridone (conjugate) was in competition with the unlabeled Fluridone (sample) for the antibody sites, the color developed is inversely proportional to the concentration of Fluridone in the sample.