ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers). The assay is validated on The BoltTM automated ELISA system.
Details
ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers).
Advantages: single replication layout and just 20 minutes of incubation time make the Celer® ELISA kit very effective screening solutions when high throughput analysis is needed.
Kit Specifications
SKU
HU0040009
Assay principle
Microplate competitive enzyme immunoassay.
Format
Microtiter plate with 96 wells
Assay time
20 min
Analysable samples
Cereals, feed, DDGS, wheat bran and middlings
Detection limit (LOD)
- Wheat, Maize, Feed adn DDGS - 0.04 ppm
- Durum Wheat - 0.12 ppm
- Wheat Bran and Middlings - 0.24 ppm
- Maize, wheat, barley, feed, DDGS: 0.04 ppm
Sample preparation
- Cereals (Maize, Wheat, Barley), Feed, DDGS - grinding, extraction in methanol-water, filtration, dilution (optional)
- Durum Wheat - grinding, water extraction, centrifugation, dilution
- Wheat Bran and Middlings - water extraction, activated charcoal clean-up, filtration, dilution
Specificity
- Deoxynivalenol 100%
- 3-acetyl-deoxynivalenol >100%
- 3-glucosyl-deoxynivalenol 64%
- 15-acetyl-deoxynivalenol 2%
- Nivalenol <4%