Immunoassay for the quantitative and sensitive screening of Azoxystrobin.
The Azoxystrobin ELISA is an immunoassay for the quantitative and sensitive screening of Azoxystrobin. This test is suitable for the quantitative and/or qualitative screening of Azoxystrobin in water or wine samples. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Azoxystrobin by specific antibodies. Azoxystrobin, when present in a sample, and an Azoxystrobin-HRP analogue competes for the binding sites of the mouse anti-Azoxystrobin antibodies in solution. The Azoxystrobin antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Azoxystrobin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.