BACGroTM Modified Tryptic Soy Broth (mTSB), when prepared as directed, is intended for the selective enrichment and isolation of pathogenic Escherichia coli strains, including E. coli O157:H7, from food matrices. This product is not intended for use in the diagnosis, treatment, or prevention of disease in humans.
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Modified Tryptic Soy Broth – abbreviated mTSB - is a nutritious, selective medium for the enrichment of pathogenic strains of E. coli, including E. coli O157:H7 and other shiga-toxin producing strains (STEC). High levels of peptones provide nutrient sources for growth promotion. Sodium chloride maintains osmotic balance of the medium. Dipotassium phosphate acts as a pH buffer. Glucose provides a carbohydrate source for fermentation processes. Bile salts provide a selective agent to inhibit the growth of Gram-positive bacteria, especially Enterococcus and Staphylococcus species.
Casein Peptone 17.0 g
Soy Peptone 3.0 g
Sodium Chloride 5.0 g
Dextrose 2.5 g
Dipotassium Phosphate 4.0 g
Bile Salts No. 3 1.5 g
Total 33.0 g/L
