Immunoassay for the quantitative and/or qualitative screening of BMAA in water samples.
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Details
The β-N-methylamino-L-alanine (BMAA) ELISA is an immunoassay for the quantitative and/or qualitative screening of BMAA in water samples. The test is a direct competitive ELISA based on the recognition of BMAA by specific antibodies. BMAA, when present in a sample, and a BMAA-HRP analog compete for the binding sites of the rabbit anti-BMAA antibodies in solution. The BMAA antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of BMAA present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
Kit Specifications
SKU
520040