Immunoassay for the quantitative and sensitive screening of Salinomycin and Narasin.
The Salinomycin/Narasin ELISA is an immunoassay for the quantitative and sensitive screening of Salinomycin and Narasin. This test is suitable for the quantitative and/or qualitative screening of Salinomycin and Narasin in animal feed and contaminated products (please refer to the appropriate technical bulletins for extraction/dilution procedures for additional matrices). Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Salinomycin and Narasin by specific antibodies. Salinomycin and Narasin, when present in a sample, and a Salinomycin-HRP analogue compete for the binding sites of rabbit anti-Salinomycin antibodies in solution. The Salinomycin antibodies are then bound by a second antibody (donkey anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Salinomycin and/or Narasin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.