PAT/pat (Liberty link) lateral flow strips (LFS) test kit is intended to be used for the qualitative detection of PAT/pat protein in individual soybean and corn leaf or seed samples. The total incubation time of the assay is 5 minutes.
Details
An antibody specific to the PAT protein molecule is immobilized on the test line area of nitrocellulose membrane.
Second antibody specific for PAT molecule is conjugated with colloidal gold and incorporated in the sample area
of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.
When the LFS is placed in the sample extract, the PAT protein present in the sample extracts binds to the
antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then
binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further
up, it binds to the control line resulting in pink/purple color control line. In absence of PAT, the test line does not
appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test
protocol.
Kit Specifications
SKU
AID 029
No. of tests
100
Max no. of samples
100
Specificity
Qualitative detection of PAT/pat protein in individual soybean and corn leaf or seed samples.
Relevant species
Individual soybean and corn leaf or seed samples.
Cross-reactivities
The PAT/Pat LFS kit does not cross react with CP4EPSPS (RoundUp Ready**), Cry1F, Cry1Ac, eCry3.1Ab
(AgriSure Duracade), Cry3Bb, Cry1Ab, Cry2A, Vip3A, Cry34Ab1 or Cry35Ab1.
Assay time
5 min
Storage
2 - 8° C
Kit components
PAT/pat LFS strips 50 strips per canister: Two canisters per kit; Extraction buffer: Two vials of 50ml each (ready to use), one pack insert, on dropper