PAT/Bar lateral flow strips (LFS) test kit is intended to be used for the qualitative detection of PAT/Bar protein in individual Mustard/Canola leaf or seed samples.
An antibody specific to the PAT/Bar protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for PAT/Bar molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.
When the LFS is placed in the sample extract, the PAT/Bar protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of PAT/Bar, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.