Cry3Bb lateral flow strips (LFS) is intended to be used for the qualitative detection of Cry3Bb protein present in transgenic corn seeds carrying Monsanto Yieldgard Root worm RW event 863. The test detects one Cry3Bb positive corn seed in 500 conventional seeds (0.2%) The total incubation time of the assay is 10 minutes.
An antibody specific to the Cry3Bb protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry3Bb molecule is conjugated with colloidal gold nano particles and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.
When the LFS is placed in the sample extract, the Cry3Bb protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry3Bb, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.