Cry3Bb lateral flow strips (LFS) is intended to be used for the qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Root worm RW event 863. The test is used to detect the Cry3Bb protein in individual Maize seed or leaf.
Details
An antibody specific to the Cry3Bb protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry3Bb molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.
When the LFS is placed in the sample extract, the Cry3Bb protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry3Bb, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.
Kit Specifications
SKU
AID 054
No. of tests
100
Max no. of samples
100
Specificity
Qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Root
worm RW event 863. The test is used to detect the Cry3Bb protein in individual Maize seed or leaf.
Relevant species
Individual Maize seed or leaf
Cross-reactivities
The Cry3Bb LFS kit does not cross react with CP4EPSPS (Roundup Ready**), Cry1F, Cry1Ac, eCry3.1Ab
(AgriSure Duracade), mCry3A, Cry1Ab, Cry2A, Vip3A, Cry34Ab1, Cry35Ab1 or PAT (LibertyLink***).
Storage
2 - 8° C.
Time to Result
5 min
Kit components
Cry3Bb LFS strips 50 strips per canister: Two canisters per kit; Extraction buffer:Two vials of 50 ml each (Ready to use); One dropper; One pack insert