Cry1Ac lateral flow strips (LFS) test kit is intended to be used for the qualitative detection of Cry1Ac protein in individual cotton leaf or seed samples. The kit detects Cry1Ac protein in Monsanto Bollgard or Bollgard II cotton. The total incubation time of the assay is 5 minutes.
An antibody specific to the Cry1Ac protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry1Ac molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS. When the LFS is placed in the sample extract, the Cry1Ac protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry1Ac, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of the test protocol.