Cry1Ac lateral flow strips (LFS) test kit is intended to be used for the qualitative detection of Cry1Ac protein in individual cotton leaf or seed samples. The kit detects Cry1Ac protein in Monsanto Bollgard or Bollgard II cotton. The total incubation time of the assay is 5 minutes.
Details
An antibody specific to the Cry1Ac protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry1Ac molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS. When the LFS is placed in the sample extract, the Cry1Ac protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry1Ac, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of the test protocol.
Kit Specifications
SKU
AID 032
No. of tests
100
Max no. of samples
100
Specificity
Qualitative detection of Cry1Ac protein in individual cotton leaf or seed samples
Relevant species
Individual Cotton leaf, seed
Time to Result
5 min
Kit components
Cry1Ac LFS strips - 50 strips per canister; Two canisters per kit, Extraction buffer - Two vials of 50 ml each (Ready to use); One Pack insert; One Calibrated dropper