Vip3A ELISA Kit

An antibody specific to the Vip3A protein molecule is immobilized on the microwell plates and the second antibody specific for Vip3A molecule is conjugated with horse radish peroxidase (HRP). When the sample extracts are added to the microtiter wells, the Vip3A from sample binds to the antibody immobilized in the well. This binding is subsequently detected by addition of an enzyme-labeled antibody. After a washing step, the substrate is added. The enzymatic reaction and development of color are proportional to the amount of Vip3A present in the sample. The reaction is terminated by the addition of stop solution. Absorbance is then measured on a plate reader.