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Cry3Bb ELISA Kit

Cry3Bb ELISA test kit is intended to be used for the qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Rootworm RW event 863. The test is used to detect the Cry3Bb protein in individual Maize seed or leaf. It can also be used to detect event 863 in bulk grain at 0.2% (1 positive in 500 negative seeds)

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Different quantities are also available in 10/50 plate formats. 
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Details
An antibody specific to the Cry3Bb protein molecule is immobilized on the microwell plates and the second antibody specific for Cry3Bb protein is conjugated with horse radish peroxidase (HRP). When the sample extracts are added to the microtiter wells, the Cry3Bb from the sample binds to the antibody immobilized in the well. This binding is subsequently detected by addition of enzyme-labeled antibody. After a washing step, the substrate is added. The enzymatic reaction and development of color are proportional to the amount of Cry3Bb present in the sample. The reaction is terminated by addition of stop solution. Absorbance is then measured on a plate reader.
Kit Specifications
SKU
AID 053
No. of tests
96
Specificity
Qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Rootworm RW event 863
Relevant species
Corn leaf, seed and bulk grain
Cross-reactivities
The Cry3Bb ELISA kit does not cross-react with CP4EPSPS (Roundup Ready**), Cry1F, Cry1Ac, eCry3.1Ab (AgriSure Duracade), mCry3A, Cry1Ab, Cry2A, Vip3A, Cry34Ab1, Cry35Ab1 or PAT (LibertyLink***)
Assay time
60 min for leaf/seed & 90 min for bulk grain
Storage
2-8 °C
Product Attachments

  • MSDS - Cry3Bb ELISA Kit AID 053

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  • Instruction for Use - AID 053 Cry3Bb ELISA

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