Immunoassay in tube format for the quantitative and sensitive screening of Microcystins and Nodularins in water samples.
The Microcystins Tube Kit is an immunoassay for the quantitative and sensitive screening of Microcystins and Nodularins in water samples. This test is suitable for the quantitative and/or qualitative screening of Microcystins and Nodularins in drinking and recreational water samples. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Microcystins, Nodularins, and their congeners by specific antibodies. The Microcystins, when present in a sample, and a Microcystins-enzyme conjugate compete for the binding sites of rabbit anti-Microcystins antibodies in solution. The Microcystins antibodies are then bound by a second antibody (anti-rabbit) immobilized on the test tube. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.