Immunoassay for the quantitative and sensitive detection of Okadaic Acid.
The Okadaic Acid ELISA is an immunoassay for the quantitative and sensitive detection of Okadaic Acid. Okadaic Acid is one of the toxins associated with diarrheic shellfish poisoning (DSP). This test is suitable for the quantitative and/or qualitative detection of Okadaic Acid in water samples as well as shellfish samples. For shellfish samples a sample preparation is required. If necessary, positive samples can be confirmed by HPLC, GC/MS, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Okadaic Acid by specific antibodies. Okadaic Acid, when present in a sample, and an okadaic acid-enzyme-conjugate compete for the binding sites of rabbit anti-okadaic acid antibodies in solution. The okadaic acid antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Okadaic Acid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.