The Abraxis Microcystins (ADDA)-DM (direct monoclonal) ELISA is an immunoassay for the quantitative and sensitive detection of Microcystins and Nodularins in water samples.
The Abraxis Microcystins (ADDA)-DM (direct monoclonal) ELISA is an immunoassay for the quantitative and sensitive detection of Microcystins and Nodularins in water samples. The test is a direct competitive ELISA for the detection of Microcystins and Nodularins. It is based on the recognition of Microcystins, Nodularins, and their congeners by a monoclonal antibody. Toxin, when present in a sample, and a Microcystins-HRP analog compete for the binding sites of anti-Microcystins antibodies in solution. The anti-Microcystins antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
No. of tests
0.1 ppb (μg/L)
1. Microtiter plate (12 X 8 strips) coated with a second antibody (goat anti-mouse) 2. Standards: 0, 0.15, 0.40, 1.0, 2.0, 5.0 ppb; 1 mL each 3. Control: 0.75 ± 0.185 ppb, 1 mL, prepared from a secondary source, for use as a Quality Control Standard (QCS) 4. Sample Diluent, 25 mL, for use as a Laboratory Reagent Blank (LRB) and for dilution of samples above the range of the standard curve 5. Microcystins-HRP Conjugate Solution, 6 mL 6. Microcystins-DM Antibody Solution (monoclonal anti-Microcystins), 6 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 16 mL 9. Stop Solution, 12 mL