The Abraxis Microcystins-ADDA SAES ELISA is an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent* detection of Microcystins and Nodularins in water samples.
The Abraxis Microcystins-ADDA SAES ELISA is an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent* detection of Microcystins and Nodularins in water samples. The test is an indirect competitive ELISA for the congener-independent detection of Microcystins and Nodularins. It is based on the recognition of Microcystins, Nodularins, and their congeners by specific biotinylated antibodies. Toxin, when present in a sample, and a Microcystins-protein analog immobilized on the plate compete for the binding sites of the biotinylated anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a streptavidin-HRP is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
No. of tests
0.016 ppb (μg/L)
1. Microtiter plate (12 X 8 strips) coated with an analog of Microcystins conjugated to a protein 2. Standards: 0, 0.05, 0.15, 0.4, 1.5, 5.0 ppb; 1 mL each 3. Control: 0.75 ± 0.185 ppb, 1 mL, prepared from a secondary source, for use as a Quality Control Standard (QCS) 4. Sample Diluent, 25 mL, for use as a Laboratory Reagent Blank (LRB) and for dilution of samples above the range of the standard curve 5. Microcystins-ADDA SAES Antibody Solution, 6 mL 6. Microcystins-ADDA SAES Conjugate Solution, 12 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 12 mL 9. Stop Solution, 6 mL