Immunoassay for the quantitative and sensitive detection of Domoic Acid (ASP).
The Domoic Acid ELISA is an immunoassay for the quantitative and sensitive detection of Domoic Acid. This kit is somewhat easier and faster than PN 520501. Domoic Acid is one of the toxins associated with amnesiac shellfish poisoning (ASP). This test is suitable for the quantitative and/or qualitative detection of Domoic Acid in water samples as well as shellfish samples. For shellfish samples a sample preparation is required. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Domoic Acid by specific antibodies. Domoic Acid, when present in a sample, and a domoic acid-enzyme-conjugate compete for the binding sites of mouse anti-domoic acid antibodies in solution. The domoic acid antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Domoic Acid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.