The Cylindrospermopsin ELISA is an immunoassay for the quantitative and sensitive detection of Cylindrospermopsin in water samples.
The Cylindrospermopsin ELISA is an immunoassay for the quantitative and sensitive detection of Cylindrospermopsin in water samples. The test is a direct competitive ELISA for the detection of Cylindrospermopsin. It is based on the recognition of Cylindrospermopsin by specific antibodies. Cylindrospermopsin, when present in a sample, and a Cylindrospermopsin-HRP analog compete for the binding sites of rabbit anti-Cylindrospermopsin antibodies in solution. The anti-Cylindrospermopsin antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Cylindrospermopsin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
No. of tests
0.04 ppb (μg/L)
1. Microtiter plate (12 X 8 strips) coated with a second antibody (goat anti-rabbit) 2. Standards: 0, 0.05, 0.10, 0.25, 0.50, 1.0, 2.0 ppb; 1 mL each 3. Control: 0.75 ± 0.15 ppb, 1 mL, prepared from a secondary source, for use as a Quality Control Standard (QCS) 4. Sample Diluent, 25 mL, for use as a Laboratory Reagent Blank (LRB) and for dilution of samples above the range of the standard curve 5. Cylindrospermopsin-HRP Conjugate Solution, 6 mL 6. Antibody Solution (rabbit anti-Cylindrospermopsin), 6 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 12 mL 9. Stop Solution, 12 mL