Immunoassay for the quantitative and sensitive detection of Brevetoxin.
The Brevetoxin ELISA is an immunoassay for the quantitative and sensitive detection of Brevetoxin. Brevetoxin is one of the toxins associated with neurotoxic shellfish poisoning (NSP). This test is suitable for the quantitative and/or qualitative detection of Brevetoxin in water samples as well as shellfish samples. Sea water samples require addition of a pretreatment solution and shellfish samples require an extraction prior to analysis. If necessary, positive samples can be confirmed by HPLC, GC/MS, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Brevetoxin by specific antibodies. Brevetoxin, when present in a sample, and a Brevetoxin enzyme-conjugate compete for the binding sites of sheep anti-Brevetoxin antibodies that have been immobilized in the wells of a microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Brevetoxin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.