BACGroTM Buffered Peptone Water (BPW), when prepared as directed, is intended for use as a nonselective pre-enrichment for Salmonella species from a wide variety of food matrices.
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All customers shall contact BACGroTM Culture Media directly via e-mail or phone +1-215-357-3911.
Various food processing steps may lead to sublethal injury of Salmonella, which subsequently may not be recovered by direct detection methods. Buffered Peptone Water allows for the recovery and growth of these organisms prior to detection. The absence of selective agents reduces stress on the cells. Unlike other pre-enrichment formulations such as Lactose Broth, BPW contains phosphate salts that provide buffering capacity to maintain the pH of the enrichment. The peptone in BPW provides a carbon and nitrogen source, and sodium chloride maintains osmotic balance. BPW is the recommended pre-enrichment medium for many food matrices in the FDA’s Bacteriological Analysis Manual (BAM)1 and the USDA’s Microbiology Laboratory Guidebook (MLG)2.
Peptone 10.0 g
Sodium Chloride 5.0 g
Sodium Phosphate, dibasic 3.5 g
Monopotassium Phosphate 1.5 g
Total 20.0 g/L
