BACGroTM Violet Red Bile Agar (VRBA), when prepared as directed, is intended for the detection and enumeration of coliform bacteria from food and dairy products. BACGroTM VRBA conforms to ISO 11133:2014.
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Violet Red Bile Agar contains peptone to provide vitamins and minerals, as well as a carbon and nitrogen source. Yeast extract promotes bacterial growth by supplying B-complex vitamins. Lactose provides a carbohydrate source. Bile salts and crystal violet aid in the inhibition of Gram-positive bacteria. Neutral red is a pH indicator in which glucose-fermenters will produce red colonies and halos (due to bile precipitation). Sodium chloride preserves osmotic balance. Agar is a solidifying agent.
VRBA may also be formulated with 4-methylumbelliferyl-β-glucuronidase (MUG) for the detection glucuronidase activity. E. coli hydrolyzes MUG with the use of the enzyme glucuronidase. The hydrolyzation of MUG produces a fluorescent compound that may be detected with UV light at 366 nm.
Peptone 7.0 g
Yeast Extract 3.0 g
Lactose 10.0 g
Bile Salts No. 3 1.5 g
Sodium Chloride 5.0 g
Neutral Red 0.03 g
Crystal Violet 0.002 g
Agar 15.0 g
Total 41.5 g/L
