BACGro Hektoen Enteric Agar / 500g

BACGroTM Hektoen Enteric Agar (HE) is a selective and differential medium used in the isolation and differentiation of Gram-negative enteric pathogens.

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Hektoen Enteric agar utilizes bile salts to inhibit Gram-positive organisms and some Gram-negative strains. Optimized differentiation is achieved through the addition of three carbohydrates: lactose, sucrose, and salicin. Ferric ammonium citrate and sodium thiosulfate allow for the detection of hydrogen sulfate production, aiding in differentiation by causing black-centered colonies for Salmonella.

Acid fuchsin and Bromothymol blue is an indicator system with a lower toxicity to promote improved enteric pathogen recovery.

Kit Specifications
Formulation (per Liter)

Animal Peptone 12.0 g

Yeast Extract 3.0 g

Bile Salts 9.0 g

Lactose 12.0 g

Sucrose 12.0 g

Salicin 2.0 g

Sodium Chloride 5.0 g

Sodium Thiosulfate 5.0 g

Ferric Ammonium Citrate 1.5 g

Bromothymol Blue 0.064 g

Acid Fuchsin 0.1 g

Agar 13.5 g

Total 75.2 g/L

2-30 °C
Coliform & E.Coli, Enterococci, Salmonella, Shigella
Product Attachments
  • Instruction for Use - Hektoen Enteric Agar

Find product documentation under Document Repository