BACGroTM MacConkey Agar w. Sorbitol (sMAC), when prepared as directed, is used as a partially selective and differential media for the isolation of E. coli O157:H7. It is not intended for use in diagnosis, treatment, or prevention of disease in humans.
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The formulation of BACGroTM sMAC is comprised of peptones to provide nutrient sources. D-sorbitol is a fermentable carbohydrate that aids in the differentiation of E. coli O157:H7 from other E. coli strains; non-fermenters (O157:H7) will remain colorless, while sorbitol-fermenters will turn pink. Bile salts and Crystal violet act as selective agents to inhibit the growth of Gram-positive organisms. Neutral red is a pH indicator.
